The World Cup victor earned a starting role for the Paris-based club today when they made the trip to the Stade des Costieres grounds to face off with the side based in Nîmes.
Marquinhos, Layvin Kurzawa and Angel Di Maria were suspended, Marco Verratti and Julian Draxler came back injured from global duty, and Mauro Icardi injured his knee in training.
'Today we have Danilo back, but he has been in contact with Cristiano Ronaldo, so the medical staff wish he did not train with the group, ' said Tuchel.
Midfielder Danilo Pereira must wait for his debut after arriving on loan from Porto - he is isolating after coming in contact with Cristiano Ronaldo, who tested positive for COVID-19, while on worldwide duty for Portugal.
"Colin Dagba had a positive (coronavirus test) with the (French) Under-21s". But they won their next four to rise to fourth behind Rennes, Lille and Lens.
With that game in mind, Tuchel said he could call on new arrivals Rafinha and Moise Kean.
"I know he'll be able to adapt quickly and of course he'll be able to play".
PSG are already missing a few players due to injuries and suspensions and one more name was added to the list on Friday night.
Kean, 20, was drafted in from Everton on loan just before the closure of the transfer window.
However, Mbappe was handed a second start in three days having scored the winning goal for France midweek in their Nations League match in Zagreb.
Around 5,000 fans will attend the game in Nimes, but other matches in France this weekend will be impacted by the government's introduction of a curfew in Paris and eight other cities as it tries to bring the ongoing coronavirus outbreak under control.
In Saturday's games, winger Hatem Ben Arfa could make his debut for Bordeaux away to Marseille.