Under new restrictions, Queensland teams will have to isolate for 14 days if they play against a Melbourne team in Queensland, play in Melbourne and then return to Queensland or play in another state against a team who has been in Melbourne in the preceding 14 days.
Australian Rules is likely to be worst affected as 10 of the 18 teams in the elite Australian Football League are based in Melbourne.
"Not unlike other teams and codes domestically and overseas, the short-term relocation of the Melbourne Rebels away from Australia's sporting capital has been a challenging and evolving situation", a Rebels statement read.
"It is a sacrifice from our club, players and staff which provides for the commencement and continuation of the 2020 Super Rugby AU competition, and illustrates our commitment to the future prosperity of rugby in Australia".
Melbourne's squad travelled to Canberra on Friday - more than a week earlier than planned - ahead of Saturday night's clash with the Brumbies with the understanding that other state governments could take similar action to Queensland's.
"By July 10, all Rebels players and staff will have been out of Victoria for the required 14 days to ensure that the Reds do not require to quarantine upon their return to Queensland after the match", a club statement read.
A Rugby Australia spokesperson had earlier told ESPN that no immediate decisions would be made over the Rebels' home games, nor those hosted by the Western Force who were hoping to return to Perth - they are now based in the NSW Hunter Valley - until more information around the COVID-19 spike in Victoria came to light.