But Prime Minister Boris Johnson has now written to her and said although he has "carefully considered and noted" her arguments, a vote would "continue the political stagnation Scotland has seen for the past decade".
"It was inevitable, but it then poses the bigger questions of the longer set of endgames that we are into", Scottish political analyst Gerry Hassan told Al Jazeera.
At the time, Sturgeon said there was an "unarguable mandate" following the SNP's election success.
Sturgeon wrote to Johnson last month asking him to enter negotiations on transferring the power to hold a referendum from London to Edinburgh.
In 2014 a referendum was held giving people in Scotland the chance to vote on whether or not they wanted to be part of the United Kingdom (England, Wales and Northern Ireland).
"Nobody realistically is thinking of unofficial referendums or a unilateral declaration of independence", said Hassan.
The renewed push for Scottish independence was a key policy for the SNP at last year's general election.
Scottish Labour's spokeswoman for climate change and the environment Claudia Beamish said: "Scotland's households account for around 15 per cent of total emissions, so it is vital the SNP government takes these warnings seriously".
"It is extremely important that in this climate emergency we in Scotland are at the forefront of tackling it".
Sturgeon and her party have vowed to hold another vote on independence, a promise which was given greater urgency after Johnson's election victory signaled that the United Kingdom would follow through with Brexit.
The spat came after Ms Sturgeon demanded that she be allowed to hold a referendum on splitting up the UK.
Pia also said that, while polling on independence remains between 45 and 50 percent, and within touching distance of a majority, it has never shown the clear majority support required to win another vote.
Scottish Liberal Democrat leader Willie Rennie also said: "The Scottish Liberal Democrats are on the side of the majority of people in Scotland do not want another independence referendum". The discontent and criticism of the [UK] government has not started building up.
Today I wrote to Nicola Sturgeon.