The rescue said their shelter building is closed for the next 30 days and warned those who have bought dogs from breeders in Iowa to test for brucellosis.
The facility and its animals are in quarantine as the dogs undergo clinical testing.
Canine brucellosis can cause reproductive failure in dogs and can be passed to humans through "direct contact or aerosol exposure to infected animal fluids", according to a fact sheet from Iowa State University.
Disease-causing brucella bacteria also can be found in the U.S.in animals like cattle, bison, elk and feral pigs.
While the risk of pet owners becoming infected with the disease is low, the health agency says those who work closely with dogs - like breeders and veterinary staff - "face an increased risk since they may be exposed to blood, tissues and fluids associated with the birthing process".
"If pet owners have recently acquired a new, small breed dog from Marion Co., they should contact their veterinarian", the department said.
Iowa Department of Agriculture officials said it's always important to practice good biosecurity, such as thoroughly washing your hands after handling animals. It requires close contact with infected dogs. "Arthritis (joint pain) and re-occurring fevers may occur with long term infection. Rarely, cases of brucellosis can involve the nervous system, eyes, or heart", reports the Center for Food Security and Public Health.
The disease can survive for months but can be eliminated with heat and disinfectants.
Amy Heinz, with De Soto-based organization AHeinz57 Pet Rescue & Transport, said 31 dogs are quarantined at her facility.
"We have not received any results yet", the organization said on Facebook.
"This is just one more reason to ADOPT and not SHOP!"