The previous two transits were by guided missile destroyer the USS McCampbell and replenishment oiler the USNS Walter S. Diehl on January 24 and the USS Stethem destroyer and the USNS Cesar Chavez cargo ship on February 25 and February 26.
The two vessels "conducted a routine Taiwan Strait transit March 24-25 ... in accordance with global law", US 7th Fleet spokesman Lt Joe Keiley said.
The U.S. Coast Guard Legend-class maritime security cutter USCGC Bertholf (WMSL 750) pulls into Joint Base Pearl Harbor-Hickam, Hawii, U.S.to support the Rim of the Pacific (RIMPAC) 2012 exercise in this June 29, 2012 handout photo.
Reuters reported the move would be viewed in Taiwan as a sign of support from Washington amid mounting friction between Taipei and Beijing. It added the USA will continue to transit by air or sea "anywhere global law allows".
The U.S. has over the past year increased its naval transits through the 180-kilometer (110 mile) wide strait that separates Taiwan from the Chinese mainland.
"The Chinese side has been closely monitoring the USA warships sailing through the Taiwan Strait", Foreign Ministry spokesman Geng Shuang said Monday.
China closely monitors the U.S.' presence in the waterway, which runs between China and Taiwan.
Meanwhile, the US Navy is continuing to conduct freedom of navigation operations in the disputed waters of the South China Sea, which Chinese authorities consider a provocation.
Taiwan President Tsai Ing-wen, to China's anger, will stop over in Hawaii this week at the end of a tour of the Pacific.
China has been ramping up pressure to assert its sovereignty over Taiwan, and has repeatedly sent military aircraft and ships to circle the nation on drills in the past few years.
Washington has no formal ties with Taiwan but is bound by law to help provide the democratic island with the means to defend itself and is its main source of arms. The Pentagon says Washington has sold Taipei more than $15 billion in weaponry since 2010.
The patrol comes days before a scheduled visit to Beijing by U.S. Treasury Secretary Steven Mnuchin and Trade Representative Robert Lighthizer, as talks on a trade agreement continue between the world's two largest economies.
Beijing has called Taiwan "the most important and sensitive issue in China-U.S. relations" and has bolstered its military presence near the island nation in recent months, sailing its sole operating aircraft carrier through the Taiwan Strait in January and March past year and holding large-scale "encirclement" exercises and bomber training throughout 2018.