It was doubly frustrating for Kohli, who had needed just eight to reach 1,000 Test runs for India in Australia.
India, the number one Test team in the world, will be begin the charge for winning their maiden Test series in Australia when the two teams face off in the first Test of the four-match series at the Adelaide Oval starting on Thursday. After the day's play former India worldwide Rohan Gavaskar expressed his disappointment at the way Murali Vijay, Rohit Sharma and Rishabh Pant got out. Pujara halted the home side's momentum with his first century in Australia, soaking up 246 balls.
He was run out attempting a quick single on the second-last ball of the day by a diving Pat Cummins.
"Playing in England, conditions are always challenging and when you come to Australia, you know that pitches are slightly better and as I said, I had decent time to prepare before the Test series", he said.
The morning got off to a awful start for Seven - the free-to-air channel somehow failed to fully capture the all-important coin toss, much to the annoyance of those watching.
Opener KL Rahul, who has been struggling for runs, flopped again, caught by Aaron Finch at third slip off Hazlewood for two.
It was stunning one-handed take by Khawaja, whose preparations were upset by the shock arrest of his brother this week for allegedly framing a love-rival with a fake terror plot.
Vice-captain Rahane (13) was then enticed into a drive with Peter Handscomb taking the catch in the slips as India lost their fourth wicket before lunch.
Rohit Sharma, preferred in the final XI to all-rounder Hanuma Vihari, compiled a patient 31 before sweeping Lyon for six, narrowly missing being caught on the ropes. Nevertheless, he played one shot too many and was dismissed by off-spinner Nathan Lyon, caught by Marcus Harris at the deep square leg region. He found willing support through Ravichandran Ashwin (25) as the pair combined for a 62-run seventh-wicket partnership.