The appeal hearing is likely to be on December 11, according to a spokesperson for the United Kingdom judiciary office.
According to CNBC, the company has filed its papers with Westminster Magistrates' Court on Friday morning.
The embattled San Francisco-based company is expected to file before the deadline, despite vows by officials in London to defend their ruling in any legal battle.
Uber's London licence expired on 30 September after TfL ruled it was not a "fit and proper" operator. Uber can still operate while the appeal is ongoing.
London mayor Sadiq Khan said on Thursday that the appeal against regulator Transport for London "will take its course" during a City Hall question time session. "We will appeal this decision on behalf of millions of Londoners, but we do so with the knowledge that we must also change".
Last week, newly-appointed Uber CEO Dara Khosrowshahi flew to London to meet with TFL. He didn't go into specifics, but TfL said it banned Uber because of the company's alleged use of its secret "Greyball" software to avoid regulators, and its approach to reporting crime. "On behalf of everyone at Uber globally, I apologize for the mistakes we've made".
The talks were described by both sides as "constructive".
Alongside the outward apologies, Uber launched a lobbying campaign to get its London customers onside. An online petition to save Uber in London has garnered more than 850,000 virtual signatures.
Beyond London, Mr Khosrowshahi seems to be trying to clean up the company's act more generally too, shaking up management, clamping down on problems around corporate culture and investigating sexual harassment claims.