Trump responded by asking, 'What's that?' the insiders said.
The amendment was formed after the assassination of President John F Kennedy in 1963.
Its four sections prescribe the procedures for the replacement of both the president and vice president's office in case of death, removal, resignation or incapacitation, respectively.
He recently told security chief Keith Schiller, 'I hate everyone in the White House!
In addition, previous members of Trump administration have gone to part ways and harshly criticise the president, the most recent of which is his former chief strategist Steve Bannon. We also slowed Trump down for tonight's edition of #DrunkDonaldTrump.
A White House official denied that Trump had made the statement.
"Yes very important you clear up that John Kelly works for the US because so much of Trump's staff works for Russia", Colbert snarked.
Once again Trump refuted the allegation via twitter saying that its was "fake news".
The amendment allows the Cabinet to remove a president if physical or mental health impairs him or her from performing the daily duties of the office.
A Google Trend result for the number of "25th Amendment" searches shows a sharp increase following the inauguration of Donald Trump.