The Jamaican great crumpled to the track with a left-leg injury as he was chasing a final gold medal for the Jamaican 4x100-meter relay team on Saturday at the world championships.
Bolt, who suffered his first 100m defeat in nine years on August 5, apparently had a pulled leg only 30 meters into the race.
Usain Bolt is unable to finish his final race as he goes down with an injury.
That was just six hundredths of a second slower than the USA's new world lead time, as Great Britain progressed as second fastest overall, with Bolt's Jamaica in third.
Olympic great Usain Bolt reacts in despair after injury cost him a relay medal at the world athletics championships in London.
Sports list five of the most memorable track races featuring Jamaican Usain Bolt, who brought down the curtain on his glittering career on Saturday.
Running the second leg, Allyson Felix and her teammates crossed first in a season's leading time of 41.84 seconds, beating Britain and Switzerland.
"He's still the man, and this is farewell so we take our hat off to him, hope he gets well soon".
Bolt beaten into third in the individual 100m behind Gatlin and his U.S. team compatriot Christian Coleman, was comforted by teammates after getting to his feet, his face etched in pain. "He is still the best in the world", Gatlin said.
Jakub Vadlejch of the Czech Republic finished second in 89.73m, ahead of fellow countryman Petr Frydrych on 88.32m.
"Two gold medals is wonderful for me", Bowie said.
In other events, Russia's Maria Lasitskene, competing as an authorized neutral athlete, retained the women's high jump title as the 24-year-old cleared 2.03 meters for the gold medal.
The victory made it a night of double relay delight for the hosts as the women's team of Asha Philip, Desiree Henry, Dina Asher-Smith and Daryll Neita won silver in 42.12s.
The Jamaican super star will call time on his illustrious career after the IAAF World Championships in London.
Eight-time Olympic champion Bolt bows out of athletics having also won 11 world gold medals but failed to add to that tally in London this summer having also surrendered his 100m crown to Justin Gatlin - a race he finished third in.